EMBO Workshop Emerging Concepts of the Neuronal Cytoskeleton 5th Edition
About the workshop

This is the fifth edition of a long-running workshop intended to expose local students and fellows to cutting edge research in the neuronal cytoskeleton field, and to help them forge closer ties with the international community that would lead to future opportunities

Cytoskeletal dynamics is at the core of the most essential questions in cellular neurobiology, such as mechanisms of axon growth in development and in response to injury, or mechanisms of synapse formation and stabilization. It is also a key element of intracellular transport, which is critical for axonal development and neurotrophic signaling, but which is also disrupted in many, if not all neurodegenerative diseases. Consequently, the cytoskeleton in neurons is a promising and emerging target for the development of novel therapeutic approaches to treat neurodegenerative diseases and to promote axon regeneration after traumatic injury. Recent advances in imaging, genetics and cell-molecular biology have catalyzed exciting new progress. However, fundamental questions about how neuronal cytoskeletal assembly, functions and dysfunction is orchestrated remain unanswered. In this workshop we will explore the latest findings on this exciting topic and will address several features involved in cytoskeleton function in nerve cells such its role in trafficking and transport, neuronal polarity, axonal guidance and neuronal migration, as microtubules amongst others. In this new edition of our workshop we will dedicate a special session to the use of super-resolution microscopy techniques in the study of neuronal functions. Also, we are very excited that in addition microtubules, actin microfilaments and neurofilaments, the role of septins, ankyrins and spectrins in neurons will be discussed.

Early career scientists are encouraged to submit abstracts to be selected for short talks.

Better Coding

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Secret to Succeess

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Make Ideas Happen

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Speakers

Claudia
Almeida

Universidade Nova de Lisboa. Portugal

Maria Elena
Avale

CONICET. Argentina

Vann
Bennett

Duke University. USA

Froylan
Calderon De Anda

Medical Center Hamburg-Eppendorf (UKE). Germany

Cecilia
Conde

Instituto Mercedes y Martin Ferreyra. Argentina

Daniel
Choquet

Interdisciplinary Institute for Neuroscience Université Bordeaux. France

Matthew
Dalva

Thomas Jefferson University USA

Erik
Dent

University of Wisconsin-Madison. USA

Shawn
Ferguson

Yale University. USA

Fiona
Francis

Inserm. France

24

Meng-meng
Fu

Stanford University. USA

Patricia
Garcez

Universidad Federal do Rio de Janeiro. Brazil

Christian
Gonzalez-Billault

Universidad de Chile Geroscience Center for Brain Health and Metabolims (GERO) Chile

Laura
Gumy

Otago University. New Zealand

Stephanie
Gupton

University of North Carolina-Chapel Hill. USA

18

Naoyuki
Inagaki

Nara Institute of Science and Technology. Japan

Sandhya
Koushika

Tata Institute of Fundamental Research. India

Christophe
Leterrier

INP, CNRS-Aix Marseille University. France

Damaris
Lorenzo

University of North Carolina-Chapel Hill, USA.

Marina
Mikhaylova

Medical Center Hamburg-Eppendorf (UKE). Germany

Kazunori
Nakajima

Keio University. Japan

Laurent
Nguyen

University of Liege. Belgium

Kassandra
Ori-Mckenney

University of California Davis. USA

Krishanu
Ray

Tata Institute of Fundamental Research. India

Subhojit
Roy

University of Wisconsin-Madison. USA

Zu-hang
Sheng

National Institute of Neurodegenerative Diseases (NINDS-NIH). USA

Monica
Sousa

Institute for Molecular and Cell Biology University of Porto. Portugal

Elias
Spiliotis

Drexel College of Medicine. USA

Alison
Twelvetrees

University of Sheffield. UK

Bettina
Winckler

University of Virgina. USA

Smita
Yadav

University of Washington. USA

James
Zheng

Emory University. USA

Workshop Program

12:00 pm - 4:00 pm

Workshop Registration


1:00 pm - 3:00 pm

Buffet-style lunch 1


3:00 pm - 4:30 pm

Hotel Check-in/Free Time


4:30 pm - 6:30 pm

SESSION 1. Super-resolution imaging in Neurons (Chair: Anthony Brown)


16:30-17:00
Christophe Leterrier,
INP, CNRS-Aix Marseille University. FR, “The axonal cytoskeleton at the nanoscale”

17:00-17:30
Marina Mikhaylova,
Medical Center Hamburg-Eppendorf (UKE). DE, “Positioning of secretory organelles in dendrites: focus on F-actin”

17:30-18:00
Monica Sousa,
Institute for Molecular and Cell Biology University of Porto. PT, “Regulation of axon diameter by the membrane periodic skeleton”

18:00-18:30
Daniel 
Choquet, Interdisciplinary Institute for Neuroscience Université Bordeaux. FR, “Linking AMPA receptor nanoscale organization and function at excitatory synapses”

6:30 pm - 7:00 pm

Break


7:00 pm - 8:00 pm

Keynote Lecture. (Chair: Subhojit Roy)


Vann Bennett, USA. “Axons as targets for human neurodevelopmental mutations: new functions for giant ankyrins”

8:15 pm

Buffet Dinner 1


9:00 am - 10:45 am

SESSION 2 Neuronal Polarity (Chair: Patricia Garcez)


9:00-9:30
Christian González-Billault, 
Universidad de Chile. CL, “Membrane and cytoskeleton dynamics coordination in neuronal polarity”

9:30-10:00
Naoyuki Inagaki,
Nara Institute for Science and Technology. JP, “Shootin1-Mediated Molecular Mechanics for Axon Guidance and Neuronal Migration”

10:00-10:30
Froylan Calderon de Anda, 
Medical Center Hamburg-Eppendorf (UKE). DE,  “Radial F-actin Organization During Early Neuronal Development”

10:30-10:45
Short talk: Sebastian Dupraz,
DZNE, Germany,“RhoA Controls Axon Extension Independent of Specification in the Developing Brain”

10:45 am - 11:15 am

Coffee Break


11:15 am - 1:15 pm

SESSION 3 Cytoskeleton in Neuronal Migration and Axonal Guidance (Chair: Stephanie Gupton)


11:15-11:45
Kazunori Nakajima,
Keio University, JP, “Neuronal migration and layer formation in the developing cerebral neocortex”

11:45-12:15
Laurent Nguyen,
University of Liege. BE, “Cell migration promotes dynamic cellular interactions to control cerebral cortex morphogenesis”

12:15-12:45
Fiona Francis,
Inserm, FR, “Cytoskeletal abnormalities affecting cortical development”

12:45-13:00
Short talk: Emily Bates;
University of Colorado, USA, “Tuba1a mutations reveal cytoskeletal interactions necessary for brain development and function”

13:00-13:15
Short talk: Maria Paz Marzolo,
Pontificia Universidad Catolica de Chile, Chile, “ApoER2, the reelin receptor, is a new cargo for the Adaptor protein AP4: implications for the exocytic trafficking and polarized sorting of ApoER2 in neurons”

 

1:00 pm - 2:30 pm

Buffet Lunch 2


2:30 pm - 5:30 pm

Free time


5:30 pm - 7:30 pm

POSTER SESSION 1


7:30 pm - 8:15 pm

Technical Lecture


Georg Wieser, Global Application Support Team, ZEISS Research Microscopy Solutions “Advanced 3D Fluorescence Live Cell Imaging Solutions for Neuronal Cytoskeletal Dynamics and Cell Motility”

8:15 pm

Buffet Dinner 2


9:00 am - 10:45 am

SESSION 4 Physiological Trafficking and Transport in Neurons (Chair: Claudia Almeida)


9:00-9:30
Bettina Winckler,
University of Virginia. USA. “Substrate-specific regulation of CDK5 activity by the intermediate filament protein nestin in neurons”

9:30-10:00
Erik Dent,
University of Wisconsin-Madison. USA. ewdent@wisc.edu  “F-BAR proteins function in actin-driven endocytosis, protrusion and neurite outgrowth”

10:00-10:30
Subhojit Roy,
University Wisconsin-Madison, USA.“Physiologic role of alpha-synuclein at the synapse”

10:30-10:45
Short talk: Francoise Coussen,
University of Bordeaux, France, “Role of synaptic plasticity in AMPA receptor intracellular trafficking”

 

10:45 am - 11:15 am

Coffee Break


11:15 am - 1:15 pm

SESSION 5 Pathological Trafficking and Transport in Neurons (Chair: Francisca Bronfman)


11:15-11:45
Sandhya Koushika,
 Tata Institute of Fundamental Research. IN, “The in vivo roles of actin in axonal cargo transport and regeneration”

11:45-12:15
Zu-hang Sheng, 
National Institute of Neurodegenerative Disease (NINDS-NIH). USA, “Microtubule-based anchoring regulates axonal mitochondrial transport in neurodegeneration and regeneration”

12:15-12:45
Claudia Almeida,
Portugal, “Actin control of neuronal endosomal sorting: implications for Alzheimer’s disease”

12:45-13:15
Shawn Ferguson,
Yale University. USA. “Axonal endo-lysosome transport mechanisms in health and disease”

1:30 pm - 3:00 pm

Buffet-style lunch 3


3:00 pm - 6:00 pm

Free time


6:00 pm - 8:00 pm

POSTER SESSION 2


8:00 pm

Buffet Dinner


9:00 am - 10:45 am

SESSION 6 Actin and Microtubules Cytoskeleton in Neurons (Chair: Elias Spiliotis)


9:00-9:30
Stephanie Gupton,
University North Carolina-Chapel Hill. USA,  “A pair of E3 ubiquitin ligases compete in axon guidance and filopodial dynamics”

9:30-10:00
Matthew Dalva, Thomas Jefferson University. USA,  “The nanoscale organization of synapses and synaptic plasticity”

10:00-10:30
James Q. Zheng, Emory University. USA,“Spatiotemporal Dynamics of Actin Monomers in Neuronal Motility”

10:30-10:45
Short talk: Marvin Bentley,
Rensselaer Polytechnic Institute,“A Novel Strategy to Visualize Vesicle-bound Kinesins Reveals Diversity of Kinesin-mediated Transport”

10:45 am - 11:15 am

Coffee Break


11:15 am - 12:45 pm

SESSION 7 Spectrin, Septins and Ankyrins in neuronal functions (Chair: Fiona Francis)


11:15-11:45 
Damaris Lorenzo, University North Carolina Chapel Hill, USA, “BII-spectrin multitasks to connect the brain”

11:45-12:15 
Elias Spilliotis, Drexel College of Medicine, USA, “Neuronal polarity requires sorting of kinesin-driven traffic during entry into dendrites by a microtubule-associated septin” 

12:15-12:30
Short talk: Robin Scharrenberg,
Center for Molecular Biology, Germany,  “Autism spectrum disorder susceptibility gene TAOK2 regulates neuronal migration in an isoform-specific manner”

1:00 pm - 2:30 pm

Buffet-style lunch 3


2:30 pm - 5:00 pm

Free time


5:00 pm - 7:00 pm

SESSION 8 “The next generation of cytoskeleton researchers”


17:00-17:15 
Patricia Garcez, Universidade Federal de Rio de Janeiro, Brazil, “CENPJ controls proliferation, migration, and axon growth through microtubule regulation”

17:15-17:30 
Laura Gumy, University of Otago, New Zealand, “MAP2-dependent regulation of axonal transport”

17:30-17:45 
Maria Elena Avale, Conicet, Argentina, “Modulation of microtubule associated protein tau isoforms: Exploring functional consequences and potential therapeutic approaches”

17:45-18:00 
Meng-meng Fu, Stanford University, USA, “The Golgi Outpost Protein TPPP Nucleates Microtubules and Regulates Branching in Oligodendrocytes”

18:00-18:15 
Alison Twelvetrees, University of Sheffield, UK, “There and back again: cooperation between dynein and kinesin motors to maintain neuronal function”

18:15-18:30 
Cecilia Conde, Instituto Mercedes y Martin Ferreyra, Argentina, “Decreased Rab11 during neuronal development disrupts dendrite growth, affecting synaptic plasticity and memory formation”

18:30-18:45
Maria M. Magiera,
Institute Curie, France,  “Perturbed microtubule polyglutamylation induces neurodegeneration in mice and humans”

18:45-19:00
Carlos Wilson,
Instituto Mercedes y Martin Ferrerya, Argentina, “Epigenetic regulation of the RhoA pathway by the histone methyl-transferase G9a promotes neuronal development”

7:15 pm - 8:00 pm

Business meeting


8:00 pm

Closing Banquet


8:30 am - 10:30 am

Breakfast and Departure


Pricing & Registration

Early Bird Registration and Abstract Submission
January 15, 2019

Registration and Abstract Submission
March 1, 2019

NOTE: Participants selected to attend the workshop will be notified within 5 business days after application

Payment deadline
March 15, 2019


To register click on create a new account, if you already have an account login here to access to web platform.

 

New account Login

US$200

Graduate and undergraduate students

US$400

Academic

US$800

Industry

US$300

Postdoctoral researchers

Poster

Posters should be printed in portrait format (90 cm wide, 120 cm long). We will provide poster boards and pins.

Registration fee waiver

Limited funds may be available to waive the registration fee for some applicants based on financial need. If you would like to be considered for this waiver, please check the box on the online application form and submit a short explanation of the financial need.

Registration includes

  • Accommodation (4 nights) on venue hotel
  • All meals, including cocktail and closing banquette. Please let us know in advance should you required any special diet.
  • All speakers will be accommodated in single rooms; students and postdocs will be accommodated in double rooms.
  • All participants will have access to all social events organized during the workshop
  • Enrollment in the workshop is limited to 120 attendees. Priority will be given to applicants that registered during early bird registration.

Payment

Payment detail will be sent to successful applicants.

Abstracts Guidelines

Abstracts should contain a title, the names and affiliations of all authors, an abstract (maximum 250 words), 3 to 4 keywords, and an acknowledgment of any funding sources.

Selection Criteria

Applicants must submit an abstract of the research that they intend to present at one of the workshop poster sessions. Applicants will be selected according to the relevance of their abstract to the workshop theme as well as the scientific quality of the research described. In addition, a small subcommittee drawn from the list of invited speakers will select student/postdoc applicants to present short talks.

Sponsors & Partners

Organizers

Christian
Gonzalez-Billault

Universidad de Chile

Patricia
Garcez

Universidad Federal do Rio de Janeiro. Brazil

Subhojit
Roy

University of Wisconsin-Madison. USA

Location and Venue

Venue
Hotel Enjoy Park Lake, Villarrica
How to get there

The nearest airport is in Temuco (Aeropuerto La Araucanía, code ZCO), which is 1.5 hours from the venue. International flights to Chile arrive to Santiago (code SCL), after which you have to take a domestic flight to ZCO. You will need about 2 hours to go through immigration and switch planes. We will organize transport from Temuco airport to the venue on the 14th, and from the venue to the airport after breakfast on the 18th.